The effect of high intensity interval exercise training on PGC-1α and ERRα gene expression in subcutaneous fat tissue of male wistar rats
Poster Presentation XML
Authors
1Student of Exercise Physiology, Sport science faculty, University of PNU, Karaj, Iran
2Assistant Professor in Exercise Physiology, Sport science faculty, University of PNU, Karaj, Iran
3Assistant Professor in Exercise Physiology, SSRI
4Associate Professor in Exercise Physiology, Sport science faculty, University of PNU, Karaj, Iran
Abstract
Adipose tissue is a highly active organ that with key roles in regulating energy balance, can be considered a treatment for many metabolic disorders and obesity. The beneficial effects of exercise training on adipose tissue and metabolic activity have been known in several studies, however, the effect of exercise intensity on molecular mechanisms that lead to changes genes transcription are largely unknown. PGC-1α and ERRα both are proteins involved in processes as mitochondrial biogenesis, browning of white adipose tissue, angiogenesis and antioxidant defense. This study aimed to investigate The effect of high intensity interval exercise training on PGC-1α and ERRα gene expression in subcutaneous fat tissue of male wistar rats.
The number of 16 male rats were divided into two groups: control groups (n=8) and high – intensity interval training (HIIT) (n=8). The training groups participated in training programs for 8 weeks. The control group did not participate in the training programme. The HIIT protocol was included 4 bouts of 4-minute activity with equivalent intensity of 90-100%- vo2max and 3 minute active rest periods and 28 Minutes of the main body. 24 hours after the final exercise session, rats was unconscious and the body of adipose tissue was extracted from the rats subcutaneous RT-PCR method was used to measure gene expression. Data analysis was performed using ANOVA and Tukey tests. The level of significance was considered as (P≤0.05.)
there was an increase in both PGC-1α and ERRα compared to the control group on Gene expression rate in HIIT trainin protocol (P<0/001).
These findings indicated that HIIT exercise training increases expression of both PGC-1α and ERRα in subcutaneous adipose tissue; so it can be used as a method to fight obesity and metabolic adaptasions and improve insulin resistance and control type 2 diabetes and fight aging.
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