The effect of endurance training along with Leucine supplementation on the serum and muscular levels of myostatin in breast tumor bearing mice
1Islamic Azad University of Science Research Tehran
2Department of physical education sciences and research Islamic azad university tehran iran
Myostatin is a secreted protein that acts as a negative regulator ofskeletal muscle mass and play important role in cancer related cachexia. The researcher in present study investigate the effect of six weeks endurance training along with Leucine supplementation on the serum and muscular levels of myostatin in breast tumor bearing mice. The 32 femalebreast tumor bearing Balb/c mice assigned in four groups including 1) control, 2) leucine, 3) training and 4) training+leucine groups (eight mice in each group). Endurance training conducted for six weeks (five session per week, 25-30 minutes per session). Leucine supplement ingested 1.35 g/kgas a daily.At the end of intervention and 48 hours after last training session or leucine ingestion, blood and muscle samples were collected and muscular and serum levels of myostatin were determined by ELISA method. Data were analyzed by one-way analysis of variance test and Tukey post-hoc test. Present findings indicated significant decrease of muscular and serum levels of myostatin in training and training+leucine groups compared to control and leucine groups.In cachexia, the quantity and quality of skeletal muscle decreases, resulting in a gradual decline in physical function, activity, independence, and quality of life. It is estimated that about 80% of cancer patients will develop cachexia during their illness and about 20% of cancer-related deaths will be cachexia. Moreover,gastrocnemius muscle weight in training and training+leucine groups were significantly higher compared to control and leucine groups (p<0.05). Decrease the myostatin levels is one of the possible pathways to combat exercise training with cancer cachexia, but leucine supplementation cannot increase the exercise trainingefficacy in mice with breast cancer.