The effect of endurance training along with Leucine supplementation on the serum and muscular levels of Follistatin and Activin a in tumor bearing mice
1Islamic Azad University, Science and Research Branch of Tehran
2Islamic Azad University of Science Research Tehran
3Physical Education, Islamic Azad University Science and Research Branch
4Department of physical education sciences and research Islamic azad university tehran iran
Cachexia is one of the main consequences of cancer which are associated with muscle mass loss.Uncontrolled weight loss due to cachexia leads to cancer patients' weakness, limiting their physical activity. In contrast, exercise training plays an important role in the fight against cachexia. The aim of present study was to investigate the effect of endurance training along with Leucine supplementation on the serum and muscular levels of Follistatin and Activin A in tumor bearing mice. In an experimental study, 32 female tumor bearing Balb/c mice assigned in 1) control, 2) leucine, 3) training and 4) training+leucine groups (eigh mice in each group). Endurance training program conducted for six weeks and five session per week on treadmill. Leucine supplementation considered 1.35 g/kg daily. At the end of six weeks intervention, blood and muscle samples were collected and Follistatin and Activin A levels were measured by ELISA method. The results indicated that muscular and serum levels of Follistatin were significantly higher in training and training+leucine groups compared to control and leucine groups (p<0.05). Muscular levels of Activin A in training and training+leucine groups compared to control and leucine groups were significantly lower (p<0.05). However, the changes in serum levels of activin A were not significant. In addition, significant decrease of tumor volume and significant increase of muscle weight in training and training+leucine groups compared to control and leucine groups were observed (p<0.05). Leucine consumption don’t affect the role of exercise training in combating cachexia and its impact on Folistatin and Activin A levels.