The Acute Effects of Endurance Training On Brain CGRP Gene Expression and Stimulation to Fat Oxidation
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Paper ID : 1472-12THCONG
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1Sport Sciences Department, Shiraz University, Shiraz, Iran
2Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Calcitonin gene-related peptide (CGRP) is a member of the calcitonin family of peptides and it is widely distributed in the central and peripheral nervous system. Serum CGRP concentration increases during exercise; however, its potential role is unclear. Recently, CGRP has been the focus of research for its lipolytic effects in vitro. The aim of the present study was to determine the effect of acute endurance training on brain CGRP gene expression in different regions of the brain and its relationship to plasma-free fatty acid (FFA) and triglyceride (TG).

20 animals were randomly divided into 2 groups including control (n = 10) and acute trained (n = 10). The trained group performed a single session of endurance training, at 26 m min-1 for 60 min, and then were killed immediately after exercise. The cortex, cerebellum, hypothalamus, hippocampus, striatum were extracted and blood sample collected. CGRP gene expression was measured by Real Time-PCR and serum concentration of CGRP, TG, and FFA were measured by ELISA technique. Statistical student t-test and Pearson correlation was used for data analysis.

In response to acute exercise, serum CGRP (P = 0.009) and plasma FFA concentration (P = 0.007) significantly increased while plasma TG was significantly decreased (P = 0.001) compared with control group. CGRP gene expression significantly increased in the cortex of the trained group (P = 0.001), but not in other parts of brain. There was a significant correlation between serum CGRP and plasma FFA (r = 0.53, p = 0.03) concentration.

In conclusion, enhanced expression of CGRP in the cortex could augment serum CGRP concentrations during endurance exercise, thereby facilitating FFA availability during exercise. These findings point out to metabolic regulation of FFA by CGRP during exercise. More researches are needed to explore how CGRP could augments fat oxidation during exercise.