Reduction of angiogenesis related factors after four training methods in left ventricle of wistar rats
Poster Presentation
Paper ID : 1427-12THCONG
Oral / Poster Presentation File: پوستر.jpg
Authors
1Master in Exercise physiology, University of Payamenoor Alborz, Iran
2professor in Exercise physiology, University of Payamenoor Alborz, Iran
3Professor in Exercise physiology, University of Payamenoor Alborz, Iran
4professor in Exercise physiology, University of Payamenoor
5استاد فیزیولوژى ورزشى کاربردى دانشگاه البرز
Abstract
Meanwhile the significance of inhibition in heart disease and lack of information about the effect of exercise training on angiogenesis related factors expression, the aim of this study was to investigate the effect of four training methods on the angiopoietin 1 and canstatin expression in left ventricle of wistar male rats.
For this purpose, 40 male wistar rats with age of 8-weeks and an average weight of 237±33 grams were randomly divided into 5 groups including control group, moderate intensity training (MIT), high intensity training (HIT), high intensity interval training (HIIT) and moderate-intensity swimming training (MIST). The MIT protocol consisted of warm-up, cooling and core exercise with 65% VO2max for 47 minutes. The HIT training protocol consisted of warm-up, cooling, and 30 minutes of core body training with 65% VO2max running at 20 m / min for 40 minutes with increasing treadmill gradient. The HIIT training protocol in the first to fourth weeks consisted of 3 intense 90-100% VO2max sprints in 4 minutes and 3 low intensity sprints with 50-60% VO2max. The MIST training protocol consisted of swimming (free swimming and staying on water) for 30 minutes. 48 hours after the last training session, cardiac tissue sampling was performed. Then, the angiopoietin 1 and canstatin expression were determined by real-time polymerase chain reaction. To analyze of the data, one way ANOVA and tukey was used at a significance level of P≤ 0.05.
The results showed that there was a significant decrease in the angiopoietin 1 and canstatin expression as a result of all four exercise training program compared to the control group (P= 0.001).
Exercise training produced favorable changes in the cardiac tissue of wistar rats in order to reduce the consequences of systemic inflammation.However, swimming exercises appear to have a more favorable effect, and further research is needed.
For this purpose, 40 male wistar rats with age of 8-weeks and an average weight of 237±33 grams were randomly divided into 5 groups including control group, moderate intensity training (MIT), high intensity training (HIT), high intensity interval training (HIIT) and moderate-intensity swimming training (MIST). The MIT protocol consisted of warm-up, cooling and core exercise with 65% VO2max for 47 minutes. The HIT training protocol consisted of warm-up, cooling, and 30 minutes of core body training with 65% VO2max running at 20 m / min for 40 minutes with increasing treadmill gradient. The HIIT training protocol in the first to fourth weeks consisted of 3 intense 90-100% VO2max sprints in 4 minutes and 3 low intensity sprints with 50-60% VO2max. The MIST training protocol consisted of swimming (free swimming and staying on water) for 30 minutes. 48 hours after the last training session, cardiac tissue sampling was performed. Then, the angiopoietin 1 and canstatin expression were determined by real-time polymerase chain reaction. To analyze of the data, one way ANOVA and tukey was used at a significance level of P≤ 0.05.
The results showed that there was a significant decrease in the angiopoietin 1 and canstatin expression as a result of all four exercise training program compared to the control group (P= 0.001).
Exercise training produced favorable changes in the cardiac tissue of wistar rats in order to reduce the consequences of systemic inflammation.However, swimming exercises appear to have a more favorable effect, and further research is needed.
Keywords
Subjects