Long and medium term swimming training- induced myocardial Gene expression
Oral Presentation
Paper ID : 1371-12THCONG
Oral / Poster Presentation File: 1371.mp4
Authors
1Department of sport sciences, Faculty of human sciences, Damghan University, Semnan, Iran
2Ph.D Student of exercise physiology, Faculty of sport sciences, Bu-Ali Sina University, Hamadan, Iran
Abstract
It seems that training for different times, have different effects on the expression of angiogenesis-related genes in the heart, but to date, the effects of long and medium term swimming training on angiogenesis has not been clearly elucidated yet.
For this purpose, the 18 male Wistar rats (210±20g), were randomly divided into three groups; control (n=6), long term (n=6), and medium term group (n=6). Training groups performed in water temperature (32 degrees Celsius) for 10 weeks (five days per week). Each session, the medium term group and the long term group performed one hour and three hour respectively. By anatomy specialist left ventricle of the heart was cuted. Real-time PCR method was used to measure the gene expression of VEGF-B, FGF-B, Ang1 & 2 and MMP2 by Ampliqon master mix and DNA synthesis kit (Denmark). Differences were determined using One-Way ANOVA and the groups were compared by Tukey follow-up test. The significance level was less than 0.05.
The expression levels of VEGF-B, Ang1 & 2 and MMP2 were significantly increased in the long-term group in compared to the both the control and medium term groups, also the genes expressed above had a significant increase in the medium term group in compared to the control group, but the gene expression of FGF-B had a significant increase only in the medium term group in compared to the control group (p=0.001).
Long-term swimming seems to be more effective in increasing the expression of angiogenic genes in the heart than in medium-term swimming.
For this purpose, the 18 male Wistar rats (210±20g), were randomly divided into three groups; control (n=6), long term (n=6), and medium term group (n=6). Training groups performed in water temperature (32 degrees Celsius) for 10 weeks (five days per week). Each session, the medium term group and the long term group performed one hour and three hour respectively. By anatomy specialist left ventricle of the heart was cuted. Real-time PCR method was used to measure the gene expression of VEGF-B, FGF-B, Ang1 & 2 and MMP2 by Ampliqon master mix and DNA synthesis kit (Denmark). Differences were determined using One-Way ANOVA and the groups were compared by Tukey follow-up test. The significance level was less than 0.05.
The expression levels of VEGF-B, Ang1 & 2 and MMP2 were significantly increased in the long-term group in compared to the both the control and medium term groups, also the genes expressed above had a significant increase in the medium term group in compared to the control group, but the gene expression of FGF-B had a significant increase only in the medium term group in compared to the control group (p=0.001).
Long-term swimming seems to be more effective in increasing the expression of angiogenic genes in the heart than in medium-term swimming.
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