effects off aerobic exercise with vitamin D consumption on AKT/mTOR signaling pathway in rat males
Oral Presentation XML
1islamic azad university central tehran branch
2hadishahr imam street
cellular and genetic adaptation induced by aerobic exercise and vitamin D consumption activated the different intracellular signaling pathway. Protein kinas B(PKB), also known as AKT and Mammalian target of rapamycin (mTOR) signaling pathway is thought to play a critical role in the regulation of protein synthesis and skeletal muscle mass. The purpose of the present study was to determine the effect aerobic exercise and vitamin D consumption on AKT/mTOR signaling pathway in muscle from rats.
In this study 50 rats (220±20 weight g ) randomly divided in to five groups of ten rats consist of: control(C), Salin (S), vitamin D(VD), training(T) and vitamin D + training (VDT). Exercise groups have been ran on treadmill four days a week in eight weeks with specific speed and10% incline and vitamin D injected to rat .05µg on daily. The total level of mTOR and AKT proteins were assessed by immunoblotting 24 hr after last exercise and in anesthetic state on soleus muscle. all data analyzed by one way ANOVA and scheffe at level of P<0.05.
total protein of mTOR and AKT were significantly increased in T and VDT groups in compared to training and control groups (P<0.05 the total AKT protein in VDT was increased in compared to training group but no significant difference between groups (P=0.078) also no significant difference in total AKT and mTOR proteins in T and VDT groups were observed (P>005).
according to the results aerobic exercise would have increased synthesis proteins in skeletal muscle mass such as AKT/mTOR pathway, if this training combined with vitamin D consumption it can probably more activated the AKT/mTOR pathway.