Comparison of the effect of 8-week training protocols on copeptin and proadrenomedoline expression in cardiac tissue of male Wistar rats
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Paper ID : 1339-12THCONG
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1universityPayam noor karaj
2Department of Education and psychology,sport science faculty,payame Noor university-karaj center, karaj , Iran
3Department of Education and psychology, Sport science faculty, Payame Noor University- Karaj center, Karaj, Iran
Copeptin is associated with individual stress levels and cardiac troponin may improve early detection of acute coronary syndrome, meanwhile mid-regional proadrenomedullin (MRproADM) is as an independent predictor in patients with coronary artery disease that transcribed by insulin, hypoxia, and several inflammatory stimuli. Considering the importance of prevention of risk factors in heart disease and lack of information about the effect of exercise training on copeptin and MRproADM expression, the aim of this study was to investigate the effect of four training methods on the copeptin and MRproADM expression in left ventricle of wistar male rats.
For this purpose, 40 male wistar rats with age of 8-weeks and an average weight of 237±33 grams were randomly divided into 5 groups including control group, moderate intensity training (MIT), high intensity training (HIT), high intensity interval training (HIIT) and moderate-intensity swimming training (MIST). The MIT protocol consisted of warm-up, cooling and core exercise with 65% VO2max for 47 minutes. The HIT training protocol consisted of warm-up, cooling, and 30 minutes of core body training with 65% VO2max running at 20 m / min for 40 minutes with increasing treadmill gradient. The HIIT training protocol in the first to fourth weeks consisted of 3 intense 90-100% VO2max sprints in 4 minutes and 3 low intensity sprints with 50-60% VO2max. The MIST training protocol consisted of swimming (free swimming and staying on water) for 30 minutes. 48 hours after the last training session, cardiac tissue sampling was performed. Then, the copeptin and MRproADM expression were determined by real-time polymerase chain reaction. To analyze of the data, one way ANOVA and bonferroni was used at a significance level of P≤ 0.05.
The results showed that there was a significant decrease in the copeptin and MRproADM expression as a result of all four exercise training program compared to the control group (P= 0.001).
Exercise training produced favorable changes in the cardiac tissue of wistar rats in order to reduce the consequences of systemic inflammation.