The Effects of Aerobic Interval Training and Caffeine consumption on Blood Markers of Lipolysis and Insulin Resistance in High Fat Diet Fed Rats

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Paper ID : 1300-12THCONG (R1)
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Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran
Caffeine, as one of the main ingredients in drinks, may be able to further suppress the risk factors associated with high-fat diet (HFD) in combination with exercise. So, the aim of this study was to investigate the effects of aerobic interval training (AIT) and caffeine consumption on plasma markers of lipolysis and insulin resistance index in rats nourished by high fat diet.
40 male Wister rats (Average age 4-6 weeks, primary weight 195±3 g) were divided into HFD and Normal diet (ND) groups. After 8 weeks of HFD or ND, HFD group was divided into 4 groups:1) control HFD, 2) HFD +AIT, 3) HFD +caffeine, 4) HFD +AIT+ caffeine. Groups of AIT with high intensity, performed 3-min bouts at 40 m/min, interspersed by 3-min active recovery at a running velocity of 20 m/min on a motorized treadmill with 15% incline, repeated six times per session (total time: 36 min). The caffeine soluble (20mg/kg) was administered daily by an oral gavage 60 minutes before activity. Normal data were analyzed with one way ANOVA and Bonferroni's post hoc test was applied.
Compared with control HFD, plasma levels of triglycerides was significantly lower in HFD +AIT, HFD +caffeine, HFD +AIT+ caffeine groups (P<0.05). Nonesterified fatty acid and glycerol concentrations did not differ between all groups. Caffeine with interval aerobic training resulted in a significant increase in plasma levels of cholesterol (P<0.05). There were significantly lower plasma glucose levels and insulin resistance index in the HFD +AIT and HFD +AIT+ caffeine groups’ vs HFD +caffeine and control HFD. In addition, plasma insulin levels was lower in HFD +AIT and HFD +AIT+ caffeine groups vs HFD +caffeine and control HFD but it was not statistically significant.
Data suggest aerobic interval training and caffeine consumption improve metabolic disorders induced by high fat diet in rats.