Effects of eight weeks of resistance training with whey protein supplementation on the rate of p70s6k and 4E-BP1 in FHL muscle in aged Wistar Rats
Poster Presentation
Paper ID : 1297-12THCONG
Authors
1Master student of Sport Physiology, Faculty of sport sciences, Ferdowsi University of Mashhad, Mashhad, Iran
2Professor of Sport Physiology, Faculty of sport sciences, Ferdowsi University of Mashhad, Mashhad, Iran
3Assistant Professor of Sport Physiology, Faculty of sport sciences, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Strength training is an effective intervention strategy to increase muscle mass, strength, and muscle function in the elderly. Several physiologically beneficial effects of consuming a whey protein isolate (WPI) have been attributed to the greater availability of bioactive peptides. The aim was to investigate the effect of eight weeks of resistance training along with whey protein supplementation on the gene expression rate of p70s6k and 4E-BP1 in flexor hallucis longus muscle in the aged rat.
In this study, 28 Wistar rats (weighing 453 ± 15) were divided into four groups: control, whey-protein supplementation, resistance training, and resistance training + whey protein supplementation. Resistance training was performed for eight weeks with a three-session exercise to climb the ladder, which included carrying 65, 85, 95 and 100 percent maximum strength abilities. Also, the 0.375 grams per kg of body weight whey protein supplement was fed daily through gavage. After surgery of the rats, FHL muscle was extracted to measure structural changes as well as the rate of gene expression in p70s6k and 4E-BP1 genes by Real-time polymerase chain reaction (real-time PCR). All data were analyzed using the non‐parametric Kruskal–Wallis one‐way ANOVA and Mann–Whitney U‐tests statistical method for comparison between groups.
The results showed a significant difference in the rate of gene expression of the p70s6k and 4E-BP1 genes between experimental groups compared to the control group after eight weeks in the FHL muscle. (p <0.001 and p <0.005, respectively).
Resistance training and whey protein intake appear to be effective ways to increase protein synthesis in old muscles.
In this study, 28 Wistar rats (weighing 453 ± 15) were divided into four groups: control, whey-protein supplementation, resistance training, and resistance training + whey protein supplementation. Resistance training was performed for eight weeks with a three-session exercise to climb the ladder, which included carrying 65, 85, 95 and 100 percent maximum strength abilities. Also, the 0.375 grams per kg of body weight whey protein supplement was fed daily through gavage. After surgery of the rats, FHL muscle was extracted to measure structural changes as well as the rate of gene expression in p70s6k and 4E-BP1 genes by Real-time polymerase chain reaction (real-time PCR). All data were analyzed using the non‐parametric Kruskal–Wallis one‐way ANOVA and Mann–Whitney U‐tests statistical method for comparison between groups.
The results showed a significant difference in the rate of gene expression of the p70s6k and 4E-BP1 genes between experimental groups compared to the control group after eight weeks in the FHL muscle. (p <0.001 and p <0.005, respectively).
Resistance training and whey protein intake appear to be effective ways to increase protein synthesis in old muscles.
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