The effect of cold water immersion after eccentric exercise on inflammation and oxidative responses in muscle tissue
Oral Presentation
Paper ID : 1110-12THCONG
Oral / Poster Presentation File: آخری.mp4
Authors
1Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran
2Department of Pharmacology, Faculty of medicine, Shahid Beheshti University of Medical Sciences,Tehran,Iran
Abstract
Exercise stress induces by eccentric exercise may be mechanical tension or metabolic stress which is caused muscle damage and increases inflammatory reaction and reactive oxygen species (ROS) production. However, the purpose of this study was to investigate the effect of immersion in cold water after eccentric exercise on inflammation and oxidative responses in muscle tissue.
100 male Wistar rat (285.11 ± 41.65) after two weeks familiarization with the environment were randomly divided into: five control groups, five eccentric exercise groups (EC), five eccentric exercise + immersion in 25°c water (EC+W25°c) and five eccentric exercise + immersion in 10°c water (EC+W10°c). The following of 0.5, 24, 48, 72 and 168 hours after eccentric exercise, animals were dissected, and Soleus muscle were removed under sterile conditions. The eccentric exercise protocol includes a 90-minute treadmill running with 16 m/min speed and -16 degree gradient slop. ROS and TNF-α were measured with DCFH-DA and immunohistochemically stained methods. Two-way ANOVA was employed to determine the significant differences between the mean values of data.
The results show that eccentric exercise has significantly increased ROS and TNF-α up to 48 hours after exercise (p<0.05). Then, these factors gradually decrease, so that there is no significant difference with the control group a week later after exercise (p>0.05). Hydrostatic pressure and cold of water have significantly increased ROS and TNF-α up to 72 hours after exercise (p<0.05). Then, these factors gradually decrease, and a week later after exercise, there are significant higher than the control group (p<0.05).
A bout of eccentric exercise increased ROS and TNF-α. It seems that, after eccentric exercise was formed a self-propelled vicious cycle between ROS and TNF-α that should be eliminate. It is possible that, after eccentric exercise, I/R injury increased this vicious cycle. Likely, immersion in cold water causes an increase in I/R injury. Thus, immersion in cold water after eccentric exercise increase this vicious cycle. Therefore, after eccentric, intensive and unaccustomed exercise, immersion in cold of water is not recommend.
100 male Wistar rat (285.11 ± 41.65) after two weeks familiarization with the environment were randomly divided into: five control groups, five eccentric exercise groups (EC), five eccentric exercise + immersion in 25°c water (EC+W25°c) and five eccentric exercise + immersion in 10°c water (EC+W10°c). The following of 0.5, 24, 48, 72 and 168 hours after eccentric exercise, animals were dissected, and Soleus muscle were removed under sterile conditions. The eccentric exercise protocol includes a 90-minute treadmill running with 16 m/min speed and -16 degree gradient slop. ROS and TNF-α were measured with DCFH-DA and immunohistochemically stained methods. Two-way ANOVA was employed to determine the significant differences between the mean values of data.
The results show that eccentric exercise has significantly increased ROS and TNF-α up to 48 hours after exercise (p<0.05). Then, these factors gradually decrease, so that there is no significant difference with the control group a week later after exercise (p>0.05). Hydrostatic pressure and cold of water have significantly increased ROS and TNF-α up to 72 hours after exercise (p<0.05). Then, these factors gradually decrease, and a week later after exercise, there are significant higher than the control group (p<0.05).
A bout of eccentric exercise increased ROS and TNF-α. It seems that, after eccentric exercise was formed a self-propelled vicious cycle between ROS and TNF-α that should be eliminate. It is possible that, after eccentric exercise, I/R injury increased this vicious cycle. Likely, immersion in cold water causes an increase in I/R injury. Thus, immersion in cold water after eccentric exercise increase this vicious cycle. Therefore, after eccentric, intensive and unaccustomed exercise, immersion in cold of water is not recommend.
Keywords
Subjects