The effect of cold water immersion on MG53 and Dysferlin proteins after eccentric exercise in muscle tissue

Poster Presentation
Paper ID : 1109-12THCONG
Oral / Poster Presentation File: Slide1.JPG 
1Department of Biological Sciences in Sport and Health, Faculty of Sports Sciences and Health, Shahid Beheshti University, Tehran, Iran
2Department of Pharmacology, Faculty of medicine, Shahid Beheshti University of Medical Sciences,Tehran,Iran
Sarcolemma repairing after exercise-induced muscle damage (EIMD) applies a series of remedial mechanism. Mitsugumin 53 (MG53) and Dysferlin (Dysf) proteins are important role in sarcolemma repairing by vesicle translocation. However, the purpose of this study was to investigate the effect of immersion in cold water on inflammation and oxidative responses, and levels of MG53 and dysferlin proteins after eccentric exercise in fast twitch and slow twitch muscles.
100 male Wistar rat (285.11 ± 41.65) after two weeks familiarization with the environment were randomly divided into: control groups, eccentric exercise groups (EC), eccentric exercise + immersion in 25°c water (EC+W25°c) and eccentric exercise + immersion in 10°c water (EC+W10°c). The following of 0.5, 24, 48, 72 and 168 hours after eccentric exercise, animals were dissected, and the soleus muscles were removed under sterile conditions. The eccentric exercise protocol includes a 90-minute treadmill running with 16 m/min speed and -16 degree gradient slop. MG53 and Dysferlin were measured with western blot method. Two-way ANOVA was employed to determine the significant differences between the mean values of data.
The results show that eccentric exercise has significantly increased MG53 and Dysferlin up to 48 hours after exercise (p<0.05). Then, these factors gradually decrease, but, 168 hours after exercise, there are significant higher than the control group (p<0.05). Hydrostatic pressure significantly increased (p<0.05) Dysferlin 0.5 hours after exercise, than, gradually decrease it, but, hydrostatic pressure has positive effect on Dysferlin. Cold of water 24 hours after eccentric exercise significantly decreased (p<0.05) Dysferlin, and until 168 hours after exercise gradually decrease Dysferlin, than, cold of water has negative effect on Dysferlin.
A bout of eccentric exercise increased MG53 and Dysferlin. It seems that, increase these proteins after eccentric exercise increase sarcolemma repairing. Overall, cold water immersion after eccentric exercise decreased MG53 and Dysferlin proteins, and it has negative effect on sarcolemma repair. Therefore, after eccentric, intensive and unaccustomed exercise, immersion in cold of water is not recommend.