The effect of high intensity interval training and berberine supplementation on creatine kinase-MB levels in rat with myocardial infarction
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Authors
1PHD student in exercise physiology, Sport sciences faculty, Bu-Ali sina university, Hamedan, Iran
2Professor in exercise physiology, Sport sciences faculty, Bu-Ali sina university, Hamedan, Iran
Abstract
Myocardial damage associated with ischemia-reperfusion injury is a major cause of mortality worldwide. One of the most important markers of myocardial infarction is creatine kinase –MB (CK-MB). The level of this marker in the bloodstream indicates the extent of damage to the myocardium. many empirical studies have shown the role high-intensity interval training (HIIT) is likely to be more effective in cardiac function protection than continuous endurance training11. In addition, berberine is an alkaloid in the form of yellow needle-shaped crystals, which is found in plants such as berberis vulgaris. Several studies have shown the effect of berberine on protection against cardiovascular diseases.
This study investigated the role of these two interventions as preconditioning factors on ck-mb levels in wistar rats one week after MI.
30 male wistar rats were divided into 3 groups of 10 (1: training, 2: berberine supplement, 3: infarct control). HIIT was performed in 8 weeks, 5 sessions per week.48 hours after the last training session, myocardial infarction (MI) was induced by descending coronary artery ligation for 30 minutes. After one week of ischemia-reperfusion, changes in the CK-MB level were measured by spectrophotometer (cecil CE 7200) on the blood plasma samples prepared with a commercial kit (Pars Azmoon, Iran).
After one week, CK-MB levels in the Intervention groups showed a significant decrease compared to the control group (p≤0.05). The CK-MB was 319 ± 2.60 in the control group and 267.96 ± 17.08, 275.95 ± 9.63 in trained and supplemented groups respectively.
To conclude, the results suggest that the use of high-intensity interval training can lead to significant changes in the myocardial level of CK-MB seven days after myocardial infarction. These findings provide a clearer insight into the reduction damage to the myocardium and improvement in patients with myocardial infarction following the adaptation to berberine consumption and high-intensity interval training program.
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