Examination of the Monoamine Oxidase A Gene Promoter on levels of voluntary Physical Activity
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Paper ID : 1072-12THCONG
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1PhD Student of Exercise Physiology, Sport Sciences Research Institute of Iran
2Payam Noor University
3Assistant Professor of Exercise Physiology, Sport Sciences Research Institute of Iran
4Faculty of Physical Education and Sport Science, Tehran University, Tehran.
Monoamine oxidase a (MAO-A) is an enzyme that acts on monoamine neurotransmitters, such as dopamine, to cause inactivation. There are several polymorphisms in the promoter region of the MAO-A gene and these variation change transcriptional activity and the amount of MAO-A produced, leading to alterations in available dopamine levels. Interestingly, polymorphisms in MAO-A have been associated recently with physical activity level. This study sought to determine whether there is an association between levels of voluntary physical activity and MAO-A gene polymorphisms.
122 participants (age 24.3 ± 5.7 years, 55 males & 67 females) completed the international physical activity questionnaire (IPAQ) to assess their level of physical activity. DNA was collected and isolated a cheek cell sample. The MAO-A genotype was identified using PCR with gene specific primers. MAO-A 3.5/3.5- 3.5/4.5 AND 4.5/4.5 Genotype individual were used for analysis.
From the sequencing results 3.5R, 4.5R and 5.5R repeats were successfully identified instead of the 3R, 4R, 5R repeats which were identified by previous researchers. In this study three variants of MAO-A - 33 subjects with 3.5/3.5 (25 men, 8 women), 25 subjects with 3.5/4.5 (2 men, 23 women), and 58 subjects with 4.5/4.5 (23 men, 34 women) - were identified. MAOA allele frequency was differ between male and women. Also, Body fat percentage and weekly MET minutes estimated by IPAQ were also comparable between genotypes.
the results suggest a polymorphism in this monoamine pathway may play a role in the regulation of physical activity and physical activity levels were higher in the 3.5/3.5 VNTR genotype compared to the 4.5/4.5 VNTR genotype.