Effect of three different exercise training (HIIT ، HIT ، MIT) on SOD and RUNX2 gene expression in the tissue of liver in Male Wistar Rats

Poster Presentation
Paper ID : 1067-12THCONG
Oral / Poster Presentation File: 1067-12THCONG.jpg 
11- Department of exercise physiology ,Student of sport science ,Payame Noor University,karaj,Iran
2Department of Education and psychology, Sport science faculty, Payame Noor University- Karaj center, Karaj, Iran
Senescence or cell aging is a process in which cells permanently stop renewing. Senescence is an irreversible damage to the cells, caused by excessive intracellular or extracellular stress or damage.
RUNX2 is a transcription factor that is a target of oxidative stress and plays an imperative role in endothelial cell function and angiogenesis. The aim of this investigate was to describes how different exercise trainings can enhance the performance of the antioxidant system and activate angiogenesis in endothelial cells of vessels in liver.
32 male Wistar rats were randomly selected and assigned in fourth groups of high-intensity interval training, HIIT(8 rats), high – intensity training, HIT (8 rats), Moderate –intensity training ,MIT(8 rats) and control group (8 rats) in a period of 8 weeks of exercise. 48 hours after the last training session, tissue samples were collected from Liver. The values of SOD and RUNX2 gene expression from tissue of liver were determined by RT-PCR. Statistical analysis was performed using SPSS software.
Our study shows that RUNX2 and SOD expressions were increased during HIIT and HIT training. The results showed that RUNX2 gene expression in both HIIT and HIT training groups in comparison to control group were significantly increased. But the SOD gene expression was significantly increased only in HIIT training.
Exercise provides changes in antioxidant defense system and angiogenesis mechanism as an anti-aging cell process in the tissue of Liver in rats. Although these effects were observed in both HIIT and HIT training, it seems that interval training has better effects on antioxidant defense system and angiogenesis.