The effect of different intensities endurance and resistance training on expression mir-103a on bone marrow in old male Aging Laboratory Rats
Paper ID : 1042-12THCONG
1Ph.D. Student in Exercise Physiology, Department of Exercise Physiology, Faculty of Literature and Humanies, Shahrekord University, Shahrekord, Iran
2Associate Professor of Sport Physiology, Department of Sport Science, Shahrekord University, Shahrekord, Iran
3Professor, Sport Physiology, Department of Sport Sciences, Shahrekord University, Shahrekord, Iran.
4Full Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
aging is known as a low bone mass and microarchitectural deterioration of bone tissue, resulting in increased risk of fracture. However, appropriate exercise for the prevention and treatment of osteoporosis has not been determined. The aim of this study was to determine the eﬀects of compare effects of aerobic and resistance exercise training protocols with different intensities on microRNA-133a (mir-133a), Runx2 and Peroxisome proliferator-activated receptors-gamma (PPARγ) in bone marrow Wistar elderly rats. This experimental study was done on 40 Wistar male rats (23 month old and with an average weight of 441.75 gram). They were randomly divided into five equal groups (n=8) include moderate (60% Maximum voluntary carrying capacity) or high (80% Maximum voluntary carrying capacity ) intensity resistance training, moderate (60-110% Maximum speed) or high (80-110% Maximum speed) intensity endurance training and control. The two training groups completed 8 weeks of training program, 5 days a week according to resistance or endurance protocols. After completing training, expression of mir-133a, Runx2 and PPARγ on bone marrow were measured RT-PCR. The statistical analysis was performed using Kruskal-Wallis test with significance level of P <0.05. There was no significance in expression of microRNA-103a and Runx2 on bone marrow were measured RT-PCR. The statistical analysis was performed using Kruskal-Wallis test with significance level of P <0.05. Results: There was no significance in expression of mir-103a (p=0.932) and Runx2 (p=0.960) in five groups. Conclusion: Investigating molecular and cellular changes in bone after such exercises in longer time can provide clearer results about the beneficial or harmful effects of these types of exercises in healthy and passive elderly people.