The Effect of Endurance Training, Detraining and Shock Training on Monocarboxylate Transporter Protein1 of Gastrocnemius Muscle and Performance in Male Wistar Rats

Poster Presentation
Paper ID : 1157-12THCONG
Oral / Poster Presentation File: Slide1.JPG 
Authors
1Masters. University of Zanjan, Faculty of Humanities, Department of Sport Sciences, Zanjan, Iran
2Assistant professor, University of Zanjan, Faculty of Humanities, Department of Sport Sciences, Zanjan, Iran
3Associate Professor, University of Zanjan , Faculty of Humanities, Department of Sport Science
Abstract
Studies have shown that aerobic exercise can have an important role in the energy metabolism of musculoskeletal system by increasing lactate transporters. However, changes in monocarboxylate transporters have been less studied during the period of detraining
In this study, 24 adult male wistar rats with Weighted average 247.05±6.75g، (8 week old) after 1 week introduction were randomly assigned in to 4 groups: control (n = 6) , endurance training (n=6) endurance training +detraining (n=6) and endurance training+shock training (n=6). The training protocol consisted of running on treadmill for 12 weeks (5 days a week), The detraining/shock training protocol (a week in a session and for 40 minutes at a speed of 20-30 m / min) was applied after one week of reduce load in the ninth week, from ten to twelve weeks. Standard meal and water were freely provided for the subjects. 48 hours after the last session of training and 4 hours of overnight fasting, serum and tissue samples were collected and Measured the level of MCT1 of Gastrocnemius tissue by ELISA method. Data were analyzed by using one way ANOVA and Tukei post hoc test.
The results showed a significant increase on MCT1 of gastrocnemius muscle in endurence training group among control groupn (P = 0.0001). but no significant changes in the level of MCT1 of gastrocnemius muscle in detraining group among endurence training (P = 1.000) and shock training (P = 0.9981) groups. a significant decrease in performance in detraining group among endurance training group (P = 0.0001). In contrast, The results showed a significant decrease in performance in the shock training group compared to the endurance training group (P = 0.0001). However, shock training keep up a significant increase in endurance performance compared to control group (P = 0.0001).
Endurance training increases the MCT1 level of the gastrocnemius muscle. This increase is not influenced by an detraining or shock training. However, shock training can significantly reduce the loss of endurance performance caused the detraining.
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